Dorothy Hudig, Ph.D.

Professor
Department of Microbiology and Immunology 320
School of Medicine
University of Nevada
Reno, NV 89557-0320

Office Phone: (775) 784-4430
Howard Medical Sceinces, Room 204
Lab Phone: (775) 784-6297
Fax: (775) 327-2332
E-mail: dhudig@medicine.nevada.edu

Research Interests:

Natural killer and T cytotoxic lymphocytes can kill tumor and virus-infected cells by means of pore-forming proteins that are released from granules of the killer lymphocytes. Granule-mediated lysis of cells is under the control of a newly discovered family of serine-dependent proteases that are termed granzymes that are bound in the granules. My laboratory was among the first to recognize the role of serine proteases in killing (1980) and is now characterizing the purified proteases, sequencing their genes, defining their natural substrates, and determining their role in lymphocyte-mediated killing.

Recent Publications:

• Hudig, D., Ewoldt, G.R., and Woodard, S.L.. "Proteases and Lymphocyte Cytotoxic Killing Mechanisms". In Innate Immunity, edited by Jay Unkeless and Sam Wright. Curr. Opin. in Immunol. 5: 90-96, 1993.
• Winkler, U., Pickett, T.M., and Hudig, D. Fractionation of perforin and granzymes using immobilized metal affinity chromatography (IMAC). J. Immunol. Methods 191:11-20, 1996.
• Ewoldt, G.R., M.J. Smyth, P.K. Darcy, J.L. Harris, C.S. Craik, B. Horowitz, S.L. Woodard, J.C. Powers, and D. Hudig. RNKP-4 and RNKP-7, new granzyme serine proteases expressed in activated rat lymphocytes. J. Immunol. 158: 4574-4583, 1997.
• Woodard, S.L., Fraser, S.A., Winkler, U., Powers, J.C., and Hudig, D. Purification and characterization of Chymase 1, a granzyme that participates in perforin lysis. J. Immunol. 160: 4988-4993, 1998.
• Fraser, S.A., Karimi, R., Michalak, M., and Hudig, D.. Perforin lytic activity is controlled by calreticulin. J. Immunol. 154 (8): 4150-4155, 2000.
• Korver, G.E., C-M Kam, J.C. Powers and D. Hudig. Dipeptide vinyl sulfones suitable for intracellular inhibition of dipeptidyl peptidase I. Int. Immunopharmacol. 1: 21-32, 2001.
• Tran, T.V., Ellis, K.A., Kam, C-M., Hudig, D., and Powers, J.C. Dipeptidyl peptidase I: Importance of progranzyme activation and other dipeptide sequences and the n-terminal amino group of synthetic substrates for enzyme activity. Accepted for publication, Arch. Biochem. Biophys., 2002.
• Hudig, D., and Karimi, R. Calreticulin in Cytotoxic Lymphocyte-Mediated Cytotoxicity In “Calreticulin”, edited by M. Michalak and P. Eggleton. Landes Bioscience, Publisher, 2002.

Search For Dr. Hudig's Publications In PubMed