Strains
The NTF (Nevada Transgenic Center) has the following transgenic lines available:
Cre-recombinase lines
Prm-cre : protamine 1 cre: sperm specific cre that is expressed in the male germ line and should be ubiquitous, total recombination
EIIa-cre : early embryo expression that can be used for partial recombination of lox sites, such as to lox out neo in 3-lox construct
MLC-2V-cre : myosin light chain: ventricle specific cre
MHC-cre : myosin heavy chain: heart specific cre (cardiac myocytes)
Tet-O-cre : tetracycline operon cre: to be used with inducible tTA (tetracycline transactivator) mice to lox out cre in a specific place upon induction
Inducible lines
MHC-AtTA : myosin heavy chain specific upon subtraction of doxycycline
Fabp1-rtTA : fatty acid binding protein specific (proximal end of small intestine) upon addition of doxycycline
Reporter line
Z/AP mice : double reporter transgenic line that expresses lacZ everywhere except where cre-mediated excision has occurred-it will then express alkaline phosphatase.