of Microbiology and Immunology 320
School of Medicine
University of Nevada
Reno, NV 89557-0320
Office Phone: (775)
Howard Medical Sceinces, Room 204
Lab Phone: (775) 784-6297
Fax: (775) 327-2332
Natural killer and T cytotoxic lymphocytes can kill tumor
and virus-infected cells by means of pore-forming proteins that are released
from granules of the killer lymphocytes. Granule-mediated lysis of cells
is under the control of a newly discovered family of serine-dependent
proteases that are termed granzymes that are bound in the granules. My
laboratory was among the first to recognize the role of serine proteases
in killing (1980) and is now characterizing the purified proteases, sequencing
their genes, defining their natural substrates, and determining their
role in lymphocyte-mediated killing.
Hudig, D., Ewoldt, G.R., and Woodard, S.L.. "Proteases
and Lymphocyte Cytotoxic Killing Mechanisms". In Innate Immunity,
edited by Jay Unkeless and Sam Wright. Curr. Opin. in Immunol. 5: 90-96,
Winkler, U., Pickett, T.M., and Hudig, D. Fractionation
of perforin and granzymes using immobilized metal affinity chromatography
(IMAC). J. Immunol. Methods 191:11-20, 1996.
Ewoldt, G.R., M.J. Smyth, P.K. Darcy, J.L. Harris, C.S.
Craik, B. Horowitz, S.L. Woodard, J.C. Powers, and D. Hudig. RNKP-4 and
RNKP-7, new granzyme serine proteases expressed in activated rat lymphocytes.
J. Immunol. 158: 4574-4583, 1997.
Woodard, S.L., Fraser, S.A., Winkler, U., Powers, J.C.,
and Hudig, D. Purification and characterization of Chymase 1, a granzyme
that participates in perforin lysis. J. Immunol. 160: 4988-4993, 1998.
Fraser, S.A., Karimi, R., Michalak, M., and Hudig, D..
Perforin lytic activity is controlled by calreticulin. J. Immunol. 154
(8): 4150-4155, 2000.
Korver, G.E., C-M Kam, J.C. Powers and D. Hudig. Dipeptide
vinyl sulfones suitable for intracellular inhibition of dipeptidyl peptidase
I. Int. Immunopharmacol. 1: 21-32, 2001.
Tran, T.V., Ellis, K.A., Kam, C-M., Hudig, D., and Powers,
J.C. Dipeptidyl peptidase I: Importance of progranzyme activation and
other dipeptide sequences and the n-terminal amino group of synthetic
substrates for enzyme activity. Accepted for publication, Arch. Biochem.
Hudig, D., and Karimi,
R. Calreticulin in Cytotoxic Lymphocyte-Mediated Cytotoxicity In “Calreticulin”,
edited by M. Michalak and P. Eggleton. Landes Bioscience, Publisher, 2002.
For Dr. Hudig's Publications In PubMed