Greg Pari, Ph.D.

Professor
Department of Microbiology and Immunology 320
School of Medicine
University of Nevada
Reno, NV 89557-0320

Office Phone: (775) 784-4824
Howard Medical Sceinces, Room 210
Lab Phone: (775) 784-1541
Fax: (775) 327-2332
E-mail: gpari@medicine.nevada.edu

Research Interests:

My laboratory is working on the molecular mechanism of herpesvirus DNA replication. We are currently working with two herpesviruses. The first, human cytomegalovirus (HCMV) is a large DNA virus with one known origin of DNA replication (oriLyt). Our interest is in the initial events in viral DNA synthesis. To this end, we are constructing mutant viruses in which a specific replication gene is deleted. This new mutant virus is then used to infect human cells to observe how the virus replicates with an essential gene missing from the genome.

The second virus we work with is a recently discovered human herpesvirus, human herpesvirus type 8 (HHV-8) also known as kaposi’s sarcoma associated herpesvirus (KSHV). This virus primarily infects B-cells where it is latent until stimulated to under go lytic replication in cell culture. Our current focus is to elucidate the cis and trans acting factors required for lytic DNA replication.

Selected Publications:

• Prichard, M. N., S. Jairath, M. E.T. Penfold, S. St. Jeor, M. C. Bohlman and G.S. Pari. 1998. Identification of a persistant RNA/DNA hybrid structures within the origin of replication (oriLyt) of human cytomegalovirus. Journal of Virology. 77:6997-7004.
• Prichard, M.N., N. Gao, S. Jairath, D.M. Coen, G. Mulamba, B.O. Parker and G.S. Pari. 1999. A recombinant human cytomegalovirus with a large deletion in the UL97 has a severe replication deficiency. Journal of Virology. 73:5663-5670.
• Abbate J, J. C. Lacayo, M. Prichard, G. Pari, and M. A. McVoy. Bifunctional protein conferring enhanced green fluorescence and puromycin resistance. Biotechniques. Vol. 31:336-340.
• Pari, G. S., D. AuCoin, K. Colletti, S. A. Cei, V. Kirchoff and S.W. Wong. 2001. Identification of a lytic origin of replication for rhesus Rhadinovirus (RRV). Journal of Virology. 75:11401-11407.
• AuCoin, D. P. and G. S. Pari. The Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) ORF 40/41 Region Encodes Two Distinct Transcripts. 2002. Journal of General Virology. 83: 189-193.
• Kirchoff, V., S. Wong, S. St. Jeor and G. S. Pari. 2002. Generation of a telomerized rhesus monkey fibroblast cell line for the growth of rhesus Rhadinovirus (RRV). Archives of Virology. 147 (2): 321 - 333.
• Chang, W. L. W., Kirchoff, V., G. S. Pari and P.A. Barry. 2002. Replication of rhesus cytomegalovirus in life-expanded rhesus diploid fibroblasts. J. Virol. Methods Jul; 104(2):135-46.
• AuCoin, D.P., Xu, Y., K. Colletti, Cei, S. and G.S. Pari. 2002. The Kaposi’s sarcoma-associated herpesvirus (HHV8) encodes two functional lytic origins. Journal of Virology. 76:7890-7896.
• Yiyang Xu, Kelly S. Colletti, and Gregory S. Pari. 2002. Human Cytomegalovirus UL84 localizes to the cell nucleus via a nuclear localization signal (NLS) and is a component of viral replication compartments. Journal of Virology. 76: 8931-8938.
• Xu, Y, K. Colletti and G.S. Pari. 2003. Human Cytomegalovirus UL84 localizes to the cell nucleus via a nuclear localization signal (NLS) and is a component of viral replication compartments. Journal of Virology. 76: 8931-8938.
• Rizvanov, A. A., van Geelen, A. G. M., Morzunov S., Otteson, E.W., Bohlman C., Pari, G.S., and S.C. St. Jeor. 2003. Generation of a Recombinant Cytomegalovirus for Expression of a Hantavirus Glycoprotein. Journal of Virology. 77:12203–12210.
• AuCoin, D.P., Colletti, K. S., Cei, S. A., Papousková, I, Tarrant M. and G. S. Pari.
  2004. Amplification of the Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Lytic Origin of DNA Replication is Dependent Upon a Cis-acting AT-Rich Region Plus an ORF50 Response Element and the Trans-acting Factors ORF50 (K-Rta) and K8 (K-bZIP). Virology. 318:542-555.
• Colletti, K. S., Xu, Y., Cei, S. A., Tarrant, M. and G. S. Pari. 2004. Expression of human cytomegalovirus UL84 oligomerization and heterodimerization domains inhibit oriLyt-dependent DNA replication: Evidence that IE2-UL84 and UL84-UL84 interactions are required for lytic DNA replication. Journal of Virology. 78:9203-9214.
• Khaiboullina S. F., Maciejewski J. P., Crapnell, K. Spallone, P. A. Dean Stock, A. Pari G. S., Zanjani E. D., Jeor, S. S. 2004. Human cytomegalovirus persists in myeloid progenitors and is passed to the myeloid progeny in a latent form. Br J Haematol. Aug;126(3):410-7.
• Xu, Y., Colletti, K. S., Cei, S. A., Tarrant, M. and G. S. Pari. 2004. A human cytomegalovirus UL84 insertion mutant is defective for viral DNA synthesis and growth. Journal of Virology. 78:10360-10369.
• Xu, Y., Colletti, K. S., Cei, S. A., Tarrant, M. and G. S. Pari. 2004. Human cytomegalovirus DNA replication requires transcriptional activation via an IE2 and UL84-responsive bidirectional promoter element within oriLyt. Journal of Virology. 78:11664-11677.
• Deyde V, Rizvanov A, Otteson E, Brandt S, Bego M, Pari G, Kozel T and St Jeor S. 2005. Identification of a monoclonal antibody from Peromyscus maniculatus (deer mouse) cytomegalovirus (PCMV) which binds to a protein with homology to the human CMV matrix protein HCMV pp71. Journal of Virological Methods. 123(1):9-15.
• Colletti, K.S., Y. Xu,, Yamboliev, I. and G. S. Pari. 2005. Human Cytomegalovirus UL84 Is a Phosphoprotein That Exhibits UTPase Activity And Is A Putative Member Of The DExD/H Box Family Of Proteins. Journal of Biological Chemistry. 280:12. 11955-11960.
• Xu, Y., AuCoin, D.P.., Huete, A.R., Cei, S.A., Hanson, L.J. and G. S. Pari. 2005. A Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 ORF50 deletion mutant is defective for reactivation of latent virus and DNA replication. Journal of Virology. 79:3479-3487.
• Bego, M., Maciejewski, J., Khaiboullina, S., Pari, G. and S. St Jeor. 2005. Novel Cytomegalovirus transcript present in monocytes of a latently infected HCMV donor. 79: 11022-11034. Journal of Virology.

 

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